Cell Metabolism
Cell Metabolism FAQs
In order to provide better customer support ,Elabscience have compiled and published the most frequently asked questions (FAQs) about cell metabolism assay kit, and will continue to update
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This kit tests for L-glutamine, not L-alanine-glutamine. L-alanine-glutamine was relatively stable in the medium, and the change of L-alanine-glutamine could not be detected by testing the medium samples with this kit.
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Using the microplate in the kit, it can be installed without spilling.There may be small differences in the maximum volume of microplate wells of different manufacturers, and overflow may occur if other microplate is used.
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The reference value of mouse serum protein concentration was 50-70g/L and the reference dilution factor was 60-80 times. Due to the differences between biological samples, the above data are for reference only.
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For samples with severe hemolysis, the effect of setting control is not obvious, it is recommended that you re-sample and use samples without hemolysis for the experiment.
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It has been documented that the probe can be co-dyed with DAPI
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The lysis buffer in this kit cannot lyse bacteria.
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This kit detects the PFK1.
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The ROS kit does not test for a specific type of ROS, but for total ROS, because the probe is not that specific.
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There is no need to specially pretreat the sample, and the conversion step is included in the actual detection process.
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It will affect the enzyme reagents, thus affecting the test results.
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Yes,it can be used in WB experiment.
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It can be increased to 40 μL~60 μL.
