Cell Metabolism
Cell Metabolism FAQs
In order to provide better customer support ,Elabscience have compiled and published the most frequently asked questions (FAQs) about cell metabolism assay kit, and will continue to update
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If the tissue weight is less than 0.1g, it can be reduced according to the proportion of tissue weight and homogenate volume as described in the manual. It is necessary to ensure that the homogenated volume of the final sample is sufficient for mitochondrial complex I detection and protein concentration detection.
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Tissue samples need to be homogenized, and the protein concentration released varies with the degree of homogenization. Therefore, by detecting the protein content in the sample, the difference in the homogenization process of the sample can be corrected to ensure the accuracy of the results. Therefore, tissue samples need to detect the protein concentration.
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Because the reaction rate of this kit is relatively fast and the control of time is very accurate, it is recommended that the number of sample wells should not exceed 8 at a time to ensure the accuracy of the measurement results. If each sample does 2 double wells, the number of samples tested at one time should not exceed 4.
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There are differences among different samples. The specific concentration and action time of reagent 1 and the specific concentration and stimulation time of reagent 2 need to be explored by pre-experiment and determined by pre-experiment.The number of samples that can be detected by the kit depends on the concentration and dosage of reagent 1.
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This kit is suitable for detecting ROS in living cells. After tissue homogenate, cells have broken and died, so this kit cannot detect tissue homogenate.If it is a fresh tissue sample, a single cell suspension is prepared and can be detected.
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It detects strong green fluorescence. If the sample carry fluorescent green there is interference, other color fluorescence has no effect on the kits.
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E-BC-F002 kit, laboratory has tested E. coli sample, is suitable.
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The probe itself is red in the kit and changes from red to green as the lipid peroxide level increases, so you can detect red fluorescence, that is, the flow cytometer selects the cy3 channel instead of the FITC channel.However, it should be noted that the probe concentration should not be too high, and it is recommended that you choose 2-3 samples for pre-experiment.
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The reagent 4 (Chromogenic Agent A ) of the kit will precipitate in the case of particularly low temperature, which is normal. Before use, incubate at 37℃ for 30 min.
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The standard is H2S and contains no other proteins. However, there are some proteins in the sample that interfere with the detection, so the sample needs to be treated with reagents 2 and 4, but the standard product is not required.So there are differences in the operation of standard curve and sample determination.
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Reagent 2 is to dissolve other precipitating substances besides ZnS.
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Reagent 4 and reagent 5 are acidic, and reagent 2 has been washed in the subsequent operation, so after adding reagent 5, is the reaction system acidic.
