Cell Metabolism
Cell Metabolism FAQs
In order to provide better customer support ,Elabscience have compiled and published the most frequently asked questions (FAQs) about cell metabolism assay kit, and will continue to update
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The homogenate medium is the same for both kits. Samples can be processed with the required reagents in either kit, and then tested separately for total iron and ferrous content,but be careful not to mix the other reagents in the two kits.
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The purpose of the control well setting is to exclude the interference of the sample itself. Each sample requires at least one control well and one sample well.
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The reagent 1 used in sample preparation will affect the determination of protein concentration, so it can not be calculated by protein concentration, but by cell number.
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The kit used the matching cell lysis buffer to process the cell samples. After adding the lysis buffer, it only needs to stand on the ice box for 10 minutes, then the supernatant can be centrifuged to be tested, which effectively improves the cell crushing efficiency.
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The concentration of indicators in the sample is relatively low, and the final color may not be judged by the eye, which is normal, but it can be detected by the instrument.
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1 King unit/100 mL = 7.14U/L
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The phenol red in cell culture medium samples can interfere with results, so this kit is not suitable for cell culture medium samples. For the detection of cell culture medium samples, the E-BC-K766-M kit is recommended.
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This kit measures the content of (Cu2+) in a sample.
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If the copper ion content in the sample exceeds the maximum detection value of the kit, the sample needs to be diluted.However, because the copper ion content in cell samples is generally relatively low, this kit does not need to be diluted to detect cell samples.
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This is a normal phenomenon, and the reagent 1 should be incubated at 37 ℃ until clear before use.
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It may be that the content of copper ions in the measured cells is low, and is lower than the detection limit of the kit. The number of sample cells can be appropriately increased to increase the concentration of copper ions in the detection sample.
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It is recommended to use fresh samples as far as possible. If the samples are frozen, the results may be low or undetectable.The longer the frozen storage time, the greater the impact on the results. Generally, the sample should not be stored at -80℃ for more than one month. If the frozen storage time is more than one month, it is not recommended to use.
