ELISPOT Kits
The ELISPOT Kit is based on the principle of the ELISA sandwich method, combined with cell culture technology. It uses pre-coated capture antibodies to capture cytokines secreted by cells, and the enzyme-labeled secondary antibody produces color spots, allowing for functional detection at the single-cell level. ELISPOT offers ultra-high sensitivity (capable of detecting active cells as rare as one in a million), single-cell resolution, visualization, and is easy and efficient to operate.
The ELISPOT Kit is an ideal tool for studying cellular immune function, precisely evaluating T/B cell activity. It is widely used in vaccine development, tumor immunotherapy, research on autoimmune disease mechanisms, and the diagnosis and efficacy monitoring of infectious diseases.
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Why Choose ELISPOT Kits
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Ultra-high Sensitivity
Capable of detecting active cells at a sensitivity of 1 in 1,000,000
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Single-cell Resolution
Direct visualization of single-cell function for precise quantification of immune activity
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High Stability
Intra- and inter-batch CV<10%, with clear and easy-to-read spots and high data reproducibility
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Ready-to-use
Pre-coated plates are easy to handle; analysis can be conducted with a microscope or plate reader
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Multi-sample Compatibility
Supports various samples such as PBMCs, spleen cells, and tumor-infiltrating lymphocytes (TILs)
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Cost-effective
Fast delivery and a cost-effective choice
Intra-and inter-batch variation testing results for various cytokines.
Detection results for the secretion of human IFN-γ and IL-1β by various cell types.
Application Case Studies
Product List
| Cat. No. | Product Name | Size |
|---|---|---|
| ESP-H0002 | Human IFN-γ (Interferon Gamma) ELISPOT Kit | 96 T*1/96 T*5 |
| ESP-H0005 | Human IL-1β (Interleukin 1β) ELISPOT Kit | 96 T*1/96 T*5 |
| ESP-H0006 | Human IL-2 (Interleukin 2) ELISPOT Kit | 96 T*1/96 T*5 |
| ESP-H0007 | Human IL-4 (Interleukin 4) ELISPOT Kit | 96 T*1/96 T*5 |
| ESP-H0008 | Human IL-5 (Interleukin 5) ELISPOT Kit | 96 T*1/96 T*5 |
| ESP-H0009 | Human IL-6 (Interleukin 6) ELISPOT Kit | 96 T*1/96 T*5 |
| ESP-H0003 | Human IL-10 (Interleukin 10) ELISPOT Kit | 96 T*1/96 T*5 |
| ESP-H0004 | Human IL-17A (Interleukin 17A) ELISPOT Kit | 96 T*1/96 T*5 |
| ESP-H0010 | Human TNF-α (Tumor Necrosis Factor Alpha) ELISPOT Kit | 96 T*1/96 T*5 |
| Cat. No. | Product Name | Size |
|---|---|---|
| ESP-M0001 | Mouse IFN-γ (Interferon Gamma) ELISPOT Kit | 96 T*1/96 T*5 |
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