Human IL-4 (Interleukin 4) ELISPOT Kit (ESP-H0007)
For research use only.
Elabscience®developed high-sensitivity ELISPOT kit, focusing on cytokine detection, to achieve single-cell level, dynamic immune function detection, ultra-high sensitivity of one per million, intuitive and reliable results.
| Size | 96T / 96T*5 |
| Manual Operation Time | 1 h |
| Assay Time | 3 h 30 min |
| Reacitivity | Human |
| applications | ELISPOT |
| Sample Volume | 100 μL |
| Sample Type | Suspension cell |
| Cross Reactivity | This kit recognizes Human IL-4 in samples.No significant cross-reactivity or interference between Human IL-4 and analogues was observed. |
| Usage | This ELISPOT kit is designed for the detection of the frequency of human IL-4 secreting cells. |
| Usage | This ELISPOT kit is designed for the detection of the frequency of human IL-4 secreting cells. |
| Precision | Both intra-CV and inter-CV are < 10%. |
| Storage | 2-8℃ |
| Expiration Date | 12 months |
| Precision | Both intra-CV and inter-CV are < 10%. |
| Background | The interleukin 4 (IL4, IL-4) is a cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. Upon activation by IL-4, Th2 cells subsequently produce additional IL-4 in a positive feedback loop. IL-4 is produced primarily by mast cells, Th2 cells, eosinophils and basophils. It is closely related and has functions similar to IL-13. Interleukin 4 has many biological roles, including the stimulation of activated B cell and T cell proliferation, and the differentiation of B cells into plasma cells. It is a key regulator in humoral and adaptive immunity. IL-4 induces B cell class switching to IgE, and up-regulates MHC class II production. IL-4 decreases the production of Th1 cells, macrophages, IFNγ, and dendritic cells IL-12.<br/> IL-4 plays an important role in the development of certain immune disorders, particularly allergies and some autoimmune diseases. |
| Uniport ID | P05112 |
| Research Area | Cell Biology , Immunology |
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