Human IFN-γ (Interferon Gamma) ELISPOT Kit (ESP-H0002)
For research use only.
Elabscience®developed high-sensitivity ELISPOT kit, focusing on cytokine detection, to achieve single-cell level, dynamic immune function detection, ultra-high sensitivity of one per million, intuitive and reliable results.
| Size | 96T / 96T*5 |
| Manual Operation Time | 1 h |
| Assay Time | 3 h 30 min |
| Reacitivity | Human |
| applications | ELISPOT |
| Sample Volume | 100 μL |
| Sample Type | Suspension cell |
| Cross Reactivity | This kit recognizes Human IFN-γ in samples.No significant cross-reactivity or interference between Human IFN-γ and analogues was observed. |
| Usage | This ELISPOT kit is designed for the detection of the frequency of human IFN-γ secreting cells. |
| Usage | This ELISPOT kit is designed for the detection of the frequency of human IFN-γ secreting cells. |
| Precision | Both intra-CV and inter-CV are < 10%. |
| Storage | 2-8℃ |
| Expiration Date | 12 months |
| Precision | Both intra-CV and inter-CV are < 10%. |
| Background | Interferon gamma (IFN-γ) is a dimerized soluble cytokine that is the only member of the type II class of interferons. Through cell signaling, IFN-γ plays a role in regulating the immune response of its target cell. A key signaling pathway that is activated by IFN-γ is the JAK-STAT signaling pathway. IFN-γ plays an important role in both innate and adaptive immunity. IFN-γ is primarily secreted by adaptive immune cells, more specifically CD4+ T helper 1 (Th1) cells, natural killer (NK) cells, and CD8+ cytotoxic T cells. The expression of IFN-γ is upregulated and downregulated by cytokines. IFN-γ expression is upregulated by the production of interleukin cytokines, such as IL-12, IL-15, IL-18, as well as type I interferons (IFN-α and IFN-β). Meanwhile, IL-4, IL-10, transforming growth factor-beta (TGF-β) and glucocorticoids are known to downregulate IFN-γ expression. By activating signaling pathways in cells such as macrophages, B cells, and CD8+ cytotoxic T cells, it is able to promote inflammation, antiviral or antibacterial activity, and cell proliferation and differentiation. IFN-γ has played a role in the development of cancer immunotherapy treatments due to its ability to prevent tumor growth.<br/> The role of IFN-γ as a disease marker has been demonstrated for a number of different pathological situations including, viral infection, Autoimmune disease, transplant rejection, Diabetes and allergy. |
| Uniport ID | P01579 |
| Research Area | Cell Biology , Immunology , Neuroscience |
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