Human TNF-α (Tumor Necrosis Factor Alpha) ELISPOT Kit (ESP-H0010)
For research use only.
Elabscience®developed high-sensitivity ELISPOT kit, focusing on cytokine detection, to achieve single-cell level, dynamic immune function detection, ultra-high sensitivity of one per million, intuitive and reliable results.
| Size | 96T / 96T*5 |
| Manual Operation Time | 1 h |
| Assay Time | 3 h 30 min |
| Reacitivity | Human |
| applications | ELISPOT |
| Sample Volume | 100 μL |
| Sample Type | Suspension cell |
| Cross Reactivity | This kit recognizes Human TNF-α in samples.No significant cross-reactivity or interference between Human TNF-α and analogues was observed. |
| Usage | This ELISPOT kit is designed for the detection of the frequency of human TNF-α secreting cells. |
| Usage | This ELISPOT kit is designed for the detection of the frequency of human TNF-α secreting cells. |
| Precision | Both intra-CV and inter-CV are < 10%. |
| Storage | 2-8℃ |
| Expiration Date | 12 months |
| Precision | Both intra-CV and inter-CV are < 10%. |
| Background | Tumor necrosis factor (TNF), formerly known as TNF-α, is a chemical messenger produced by the immune system that induces inflammation. TNF is produced primarily by activated macrophages, and induces inflammation by binding to its receptors on other cells. It is a member of the tumor necrosis factor superfamily, a family of transmembrane proteins that are cytokines, chemical messengers of the immune system. Excessive production of TNF plays a critical role in several inflammatory diseases, and TNF-blocking drugs are often employed to treat these diseases. <br/> TNF is produced primarily by macrophages but is also produced in several other cell types, such as T cells, B cells, dendritic cells, and mast cells. It is produced rapidly in response to pathogens, cytokines, and environmental stressors. TNF is initially produced as a type II transmembrane protein (tmTNF), which is then cleaved by TNF alpha converting enzyme (TACE) into a soluble form (sTNF) and secreted from the cell. Three TNF molecules assemble together to form an active homotrimer, whereas individual TNF molecules are inert. |
| Uniport ID | P01375 |
| Research Area | Cell Biology , Immunology |
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