If the sample is hemolysis, can interference be eliminated by setting controls?

It depends on the degree of hemolysis.If it's not serious, you don't need to set a control. It doesn't have much effect. If hemolysis is severe, it is not recommended to test, even if the control is set, it may have a greater impact on the result.

Can the high concentration of hydrochloric acid in the kit be replaced by other reagents?

It can't be replaced with any other reagent, you need to use concentrated hydrochloric acid.

What is the recommended dilution of mouse skin tissue?

The reference dilution of mouse skin tissue was about 20-30 times. The dilution ratio is for reference only. Due to the differences among biological samples, it is recommended to select several samples for pre-experiment to determine the best dilution ratio.

What is the effect of sample supernatant treated with reagent 4 working solution?

In order to eliminate the interference substances of the sample itself and ensure the accuracy of the results.

The tissue sample has been homogenized with normal saline. Will it affect the result?

For this kit, tissue samples need to be homogenized with powder A application solution to ensure the MPO extraction effect. If normal saline is homogenized, the measured value may be affected and the result may be low.

What might be the cause of turbidity when testing serum samples? How should it be adjusted?

It may be due to the protein precipitation in the sample, it is recommended to increase the dilution ratio of the sample before testing.

For cell culture medium samples, does it matter if there is phenol red in the culture medium?

There is not much impact, you can set the control well to see the measured value, if the control well OD value is lower than the blank, you can not set the control.

Does the kit measure total or free calcium content?

This kit detects the content of free calcium in the sample.

Does the kit detect L-glutamic acid or total glutamic acid?

This kit detects the content of L-glutamic acid in the sample.

Can the glacial acetic acid in E-BC-K025-M kit be replaced with hydrochloric acid?

No, you can't substitute for two acids in different concentrations.

What is the purpose of determining the OD value A1 for the first time after adding reagent 2 to the kit for 2min? Why do I need to calculate the difference between the two measurements?

Since the sample itself contains creatinine and creatine, the addition of reagent 1 converts the creatine in the sample to hydrogen peroxide. But the creatinine in the sample did not react at the first step, but from the second step; The reaction in the first 2 min is to consume the creatine contained in the sample itself, and this part of the measurement value is deducted to ensure the accuracy of the measurement results.

If the drug used in the experimental treatment is an iron chelating agent, and the kit principle is also an iron chelating agent, will there be a competitive relationship that will lead to changes in the test results?

If the purpose of the experiment is to verify the effect of the drug (iron chelating agent), it can be detected with this kit without interference. The probe of this kit is to detect iron ions that have not been chelated, and the probe will not rob the iron that has been chelated.

Cell Metabolism

Cell Metabolism FAQs

In order to provide better customer support ,Elabscience have compiled and published the most frequently asked questions (FAQs) about cell metabolism assay kit, and will continue to update

It depends on the degree of hemolysis.If it's not serious, you don't need to set a control. It doesn't have much effect. If hemolysis is severe, it is not recommended to test, even if the control is set, it may have a greater impact on the result.
It can't be replaced with any other reagent, you need to use concentrated hydrochloric acid.
The reference dilution of mouse skin tissue was about 20-30 times. The dilution ratio is for reference only. Due to the differences among biological samples, it is recommended to select several samples for pre-experiment to determine the best dilution ratio.
In order to eliminate the interference substances of the sample itself and ensure the accuracy of the results.
For this kit, tissue samples need to be homogenized with powder A application solution to ensure the MPO extraction effect. If normal saline is homogenized, the measured value may be affected and the result may be low.
It may be due to the protein precipitation in the sample, it is recommended to increase the dilution ratio of the sample before testing.
There is not much impact, you can set the control well to see the measured value, if the control well OD value is lower than the blank, you can not set the control.
This kit detects the content of free calcium in the sample.
This kit detects the content of L-glutamic acid in the sample.
No, you can't substitute for two acids in different concentrations.
Since the sample itself contains creatinine and creatine, the addition of reagent 1 converts the creatine in the sample to hydrogen peroxide. But the creatinine in the sample did not react at the first step, but from the second step; The reaction in the first 2 min is to consume the creatine contained in the sample itself, and this part of the measurement value is deducted to ensure the accuracy of the measurement results.
If the purpose of the experiment is to verify the effect of the drug (iron chelating agent), it can be detected with this kit without interference. The probe of this kit is to detect iron ions that have not been chelated, and the probe will not rob the iron that has been chelated.

All categories

Cell Metabolism

Cell Metabolism

Cell Metabolism FAQs

Q1. If the sample is hemolysis, can interference be eliminated by setting controls?

Q2. Can the high concentration of hydrochloric acid in the kit be replaced by other reagents?

Q3. What is the recommended dilution of mouse skin tissue?

Q4. What is the effect of sample supernatant treated with reagent 4 working solution?

Q5. The tissue sample has been homogenized with normal saline. Will it affect the result?

Q6. What might be the cause of turbidity when testing serum samples? How should it be adjusted?

Q7. For cell culture medium samples, does it matter if there is phenol red in the culture medium?

Q8. Does the kit measure total or free calcium content?

Q9. Does the kit detect L-glutamic acid or total glutamic acid?

Q10. Can the glacial acetic acid in E-BC-K025-M kit be replaced with hydrochloric acid?

Q11. What is the purpose of determining the OD value A1 for the first time after adding reagent 2 to the kit for 2min? Why do I need to calculate the difference between the two measurements?

Q12. If the drug used in the experimental treatment is an iron chelating agent, and the kit principle is also an iron chelating agent, will there be a competitive relationship that will lead to changes in the test results?