Antibody & Protein
Antibody & Protein FAQs
Elabscience® has compiled a list of the most frequently asked questions (FAQs) about the application of our antibody & protein products, with the goal of providing more efficient technical support to our customers.
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Polyformaldehyde (PFA) is a commonly used tissue fixative, typically used to immobilize proteins and cellular structures in biological samples. Although PFA can help maintain cell structure and protein morphology, PFA fixation may cause damage to the cell membrane due to its permeability, which can fix proteins inside and outside the cell. Additionally, the permeability of different cells may not be consistent. The appropriate fixative should be selected based on which aspect of research will be used for the cell membrane.
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The occurrence of foam on the cell membrane may be due to prolonged fixation time, and the fixation time can be appropriately shortened to optimize the fixation results.
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Polyformaldehyde is prone to volatilization, so it should be stored in a low temperature sealed and dark place.
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When using this product to prepare TBST, the final concentration of Tween should be controlled at 0.1%.
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This product can be directly used for dyeing without dilution.
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If blue precipitate is found in the dye solution before the experiment, filter it with filter paper before use. Whether the dye solution is ineffective can only be judged based on the staining results.
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When using Triton X-100 to lyse cells, the concentration is generally 1%; PBS or TBS can be used for dilution.
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This product does not contain sodium azide. You can add it yourself if needed.
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All are compatible.
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It may be difficult to decolorize because impurities in gel are not removed completely. It can be improved by timing the replacement of deionized water, extending the decolorization time and appropriately increasing the decolorization temperature during the decolorization process.
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This product can be reused 2-3 times.
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By default, the goods are delivered as mentioned in the manual; If you have special needs, it needs to be determined according to the protein situation. In principle, lyophilized powder protein can be sent in liquid form, how liquid protein cannot be sent in ly powder form.
