Antibody & Protein FAQs

Room temperature storage will have a certain impact on the effectiveness of the product, and the usability of the product can be verified through pre experiments.

Boiling will have a certain impact on the effectiveness of the product, and the usability of the product can be verified through pre experiments.

The loading buffer will have an impact on the electrophoresis solution. If the overflow is small, it can be ignored. If there is a large overflow, it is necessary to replace the electrophoresis solution with fresh one in a timely manner to avoid affecting the subsequent electrophoresis effect. To avoid buffer overflow, it is recommended to dissolve completely in a temperature bath below 37 ℃ before use.

It is not recommended to perform such an operation. Boiling the protein is difficult to completely mix with the buffer, which affects the electrophoresis effect. Mixing the protein with the buffer before boiling is also beneficial for protein denaturation. When in use, the protein is usually thoroughly mixed with the loading buffer before boiling for denaturation.

If a precipitate is found in the sample buffer, it can be dissolved at room temperature or 37 ℃. After the precipitate is completely dissolved, it can continue to be used. When dissolving, do not immerse in a water bath for a long time or boil directly to avoid the deterioration of certain components in the product.

It will not have any impact. SDS in the buffer can bind to proteins and cause the protein SDS complex to carry a large amount of negative charge, completely covering the protein's own charge.

The denatured sample can be diluted with a buffer solution diluted to 1 ×.

If the protein sample contains a relatively high amount of dimers, a reducing agent DTT can be added to help open the dimers.

If the protein band movement rate is uneven, it is recommended to completely dissolve the loading buffer in a bath below 37 ℃ before adding the protein sample. Mix it evenly with the protein sample and boil for 10 minutes to completely denature the protein sample. If the protein band moves slowly, it is necessary to promptly check whether there is any leakage in the electrophoresis chamber.

The precipitation of denatured protein buffer may be related to the ion concentration in the protein sample. Precipitation occurs during temperature changes and can be dissolved by incubating in a water bath below 37 ℃. If there is still a small amount of sediment that cannot be dissolved, centrifuge and take the supernatant for use.

100℃。