Antibody & Protein FAQs

This product has no significant effect on its effectiveness when left at room temperature (around 25 ℃) for 24 hours and can continue to be used. But it is recommended to immediately pack the goods and store them at -20 ℃, with no more than 5 repeated freeze-thaw cycles.

This product can extract mitochondrial proteins.

When using this product, the removal solution can be restored to room temperature before use; The same membrane can be cleared of antibodies 1-2 times, usually once is recommended.

DAPI can be excited using excitation light at 405 nanometers. Although the optimal excitation wavelength for DAPI is in the ultraviolet region (approximately 350-360 nanometers), it can also effectively excite under blue light at 405 nanometers. Using a 405 nanometer excitation light source can still activate DAPI and generate blue fluorescence for observing and imaging cell nuclei.

This product is only suitable for staining fixed cells. It is not recommended to directly stain live cells or use DAPI staining to determine cell apoptosis. To assess cell apoptosis, it is recommended to use specialized detection kits such as TUNEL, Annexin V-FITC, etc.

This product can be directly diluted to the working solution concentration according to the instructions during use, without the need for additional pH adjustment.

Not recommended for recycling.

This product contains a buffer salt solution, which will not cause significant changes in pH value when diluted from a concentrated solution to a working solution, and there is no need to adjust the pH value again.

The effective pH range of proteinase K is pH 4.0-12.5, and the optimal pH range is pH 7.5-8.0. Please use a buffer solution with the appropriate pH mentioned above, such as 10mM Tris HCl pH 7.4-7.8, to prepare the proteinase K solution according to the experimental requirements. The commonly used working concentration of proteinase K is 10-100ug/ml, and the storage solution concentration is generally 100-200mg/ml. It should be stored at -20 ℃ and diluted to the working concentration before use to avoid prolonged storage.

No need.

Normally, after fixation in 4% paraformaldehyde, tumor tissue slices can be stored at room temperature for weeks to months. For long-term preservation, a better approach is to transfer the fixed tissue samples to a liquid containing buffer such as PBS and store them in a refrigerator at 4 degrees Celsius to ensure their stability and suitability.