Pyruvate Fluorometric Assay Kit (E-BC-F058)

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All	Size	Price	Qty
	96T	$ 620.00
	48T	$ 450.00

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Detection Principle	Pyruvate is an important component of the organism. It mainly participates in the metabolism of sugar and fat, and is also one of the intermediate products of carbohydrate metabolism. The detection principle of this kit is that pyruvate undergoes a series of reactions under the action of enzyme reagents, and the resulting substance can react with the color developer. The content of pyruvate in the sample is calculated by measuring the fluorescence value of excitation wavelength 535 nm and emission wavelength 587 nm.
Sample Type	Serum,Plasma,Urine,Animal tissue,Cell
Detection Method	Fluorometric method
Detection Instrument	Fluorescence microplate reader (Ex/Em=535 nm/587 nm)
Research Area	Glycolysis And Lipid Metabolism , Tricarboxylic Acid（TCA）Cycle , Energy Metabolism
Other Reagents Required	Normal saline (0.9% NaCl)
Storage	This product can be stored at -20°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	1.00 μmol/L
Detection Range	1.00-100 μmol/L
Precision	inter-assay CV: 2.0-4.0% \| intra-assay CV: 1.0-2.0%
Sample Volume	100-150 μL
Assay Time	40 min

Cat.No.	Product Name	Sizes
E-BC-F046	Acetyl Coenzyme A (Acetyl-CoA) Fluorometric Assay Kit	96T , 48T
E-BC-F047	α-Ketoglutarate (α-KG) Fluorometric Assay Kit	96T , 48T
E-BC-K083-M	α-Ketoglutarate Dehydrogenase (α-KGDH) Activity Assay Kit	96T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
Guinea pig serum	1
Rabbit serum	1-2
Human serum	1
Rat serum	1-2
10% Mouse kidney tissue homogenate	1
10% Mouse liver tissue homogenate	1
1×10^6 239T cells	1
1×10^6 CHO cells	1
1×10^6 Hela cells	1
1×10^6 Jurkat cells	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.