α-Ketoglutarate (α-KG) Fluorometric Assay Kit (E-BC-F047)
For research use only.
| Detection Principle | α-Ketoglutaric acid and alanine can be combined with fluorescent probe products under the action of a series of enzymes. The content of α-ketoglutaric acid in samples can be determined by measuring the fluorescence value. |
| Synonyms | α-KG |
| Sample Type | Serum,Plasma,Urine,Animal tissue,Cell sample |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
| Research Area | Tricarboxylic Acid（TCA）Cycle , Cuproptosis , Energy Metabolism |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.60 μmol/L |
| Detection Range | 0.60-100 μmol/L |
| Precision | inter-assay CV: 0.04 | intra-assay CV: 0.02 |
| Sample Volume | 20 μL |
| Assay Time | 35 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Rat kidney tissue homogenate | 1 |
| 10% Rat lung tissue homogenate | 1 |
| 10% Porcine heart tissue homogenate | 1 |
| 10% Rat brain tissue homogenate | 1 |
| 10% Mouse liver tissue homogenate | 1 |
| 10^6 Jurkat cell | 1 |
| Human serum | 1 |
| Rat serum | 2-3 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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