α-Ketoglutarate Dehydrogenase (α-KGDH) Activity Assay Kit (E-BC-K083-M)
For research use only.
| Detection Principle | α-ketoglutarate dehydrogenase (α-KGDH) is a key enzyme in the tricarboxylic acid (TCA) cycle. While the substrate being catalyzed by α-KGDH, NAD+ is reduced to NADH, and electrons are transfered to WST-8 under the action of hydrogen transmitter to produce yellow product. The activity of α-KGDH can be calculated by measuring the change of absorbance value at 450 nm |
| Synonyms | α-KGDH |
| Sample Type | Serum,Plasma,Animal tissue,Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (440-460 nm, optimum wavelength: 450 nm) |
| Research Area | Mitochondria Function , Tricarboxylic Acid（TCA）Cycle , Energy Metabolism |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 8.3 U/L |
| Detection Range | 8.3-42.3 U/L |
| Precision | inter-assay CV: 8.5% | intra-assay CV: 1.5% |
| Sample Volume | 40 μL |
| Assay Time | 40 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Rat liver tissue homogenate | 1 |
| 10% Rat lung tissue homogenate | 1 |
| 10% Mouse liver tissue homogenate | 1 |
| 10% Epipremnum aureum tissue homogenate | 1 |
| 10% Mouse spleen tissue homogenate | 1 |
| Rat plasma | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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