Acetyl Coenzyme A (Acetyl-CoA) Fluorometric Assay Kit (E-BC-F046)
For research use only.
| Detection Principle |
Acetyl-CoA is an essential cofactor and acyl carrier in the enzymatic acetyl transfer reaction. It can be formed by oxidative decarboxylation of pyruvate in mitochondria, oxidation of long-chain fatty acids, or oxidative degradation of certain amino acids. Acetyl-CoA is the starting compound of the citric acid cycle. The detection principle of this kit is that Acetyl-CoA is converted into other substances under the condition of enzyme catalysis, and the resulting fluorescence is detected by fluorescence microplate reader at the maximum excitation wavelength of 535 nm and the maximum emission wavelength of 587 nm. |
| Sample Type | Serum,Plasma,Animal tissue,Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
| Research Area | Tricarboxylic Acid（TCA）Cycle , Energy Metabolism |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.27 μmol/L |
| Detection Range | 0.27-5.00 μmol/L |
| Precision | inter-assay CV: 2.7-3.6% | intra-assay CV: 1.7-4.1% |
| Sample Volume | 200-400 μL |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse heart tissue homogenate | 1 |
| 10% Mouse liver tissue homogenate | 1 |
| 10% Mouse lung tissue homogenate | 1 |
| 10% Mouse kidney tissue homogenate | 1 |
| Mouse plasma | 1 |
| Rat serum | 1 |
| Rat plasma | 1 |
| 1×10^6 CHO cells | 1 |
| 1×10^6 Molt-4 cells | 1 |
| 1×10^6 HL-60 cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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