Fumarase (FUM) Activity Colorimetric Assay Kit (E-BC-K908-M)
For research use only.
| Detection Principle |
Fumarase (FUM), also known as malate lyase, fumarate hydrase or fumarase, is an important enzyme in the tricarboxylic acid cycle, which can catalyze the hydration of fumarase to synthesize malic acid. FUM is a kind of intracellular enzyme that exists widely in plants and animals. The detection principle of this kit: fumarase catalyze fumaric acid products under the action of electronic coupler to reduce the chromogenic reagent to orange products, detection at about 450 nm has the maximum absorption peak. |
| Sample Type | Serum,Plasma,Animal tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (445-465 nm, optimum wavelength: 450 nm) |
| Research Area | Tricarboxylic Acid（TCA）Cycle |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.696 U/L |
| Detection Range | 0.696-33.106 U/L |
| Precision | inter-assay CV: 5.8-7.7% | intra-assay CV: 2.4-5.0% |
| Sample Volume | 40 μL |
| Assay Time | 50 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenization | 200-300 |
| 10% Mouse kidney tissue homogenization | 200-300 |
| 10% Mouse heart tissue homogenization | 200-300 |
| 10% Mouse lung tissue homogenization | 200-300 |
| Rat plasma | 2-4 |
| Mouse serum | 2-4 |
| Mouse plasma | 2-4 |
| Human serum | 2-4 |
| 1×10^6 293T cells | 2-4 |
| 1×10^6 Hela cells | 2-4 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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