Succinic Acid Colorimetric Assay Kit (E-BC-K902-M)

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	96T	$ 670.00

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Detection Principle	Succinic acid, also known as succinic acid, is widely present in all plant and animal tissues, was first extracted from amber, is an intermediate in the citric acid cycle, and plays an important role in the production of energy in cells. Succinate, or succinate, is widely used in the agricultural, food and pharmaceutical industries due to its low toxicity. Succinic acid is catalyzed by enzyme reagent to form colored substance with color developer. The maximum absorption is at 555 nm. The content of succinic acid in samples can be calculated by measuring OD value and standard curve at 555 nm.
Sample Type	Serum,Plasma,Animal tissue,Cell
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (550-560 nm, optimum wavelength: 555 nm)
Research Area	Tricarboxylic Acid（TCA）Cycle , Energy Metabolism
Storage	This product can be stored at -20°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	0.007 mmol/L
Detection Range	0.007-0.5 mmol/L
Precision	inter-assay CV: 3.4-9.3% \| intra-assay CV: 3.0-6.0%
Sample Volume	40 μL
Assay Time	1 h 10 min

Cat.No.	Product Name	Sizes
E-BC-F047	α-Ketoglutarate (α-KG) Fluorometric Assay Kit	96T , 48T
E-BC-K083-M	α-Ketoglutarate Dehydrogenase (α-KGDH) Activity Assay Kit	96T
E-BC-K130-M	Pyruvic Acid Colorimetric Assay Kit	96T , 48T
E-BC-K554-M	NADP-Isocitrate Dehydrogenase (NADP-IDH) Activity Assay Kit	96T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
10% Mouse liver tissue homogenate	1
10% Mouse kidney tissue homogenate	1
10% Rats liver tissue homogenate	1
10% Rats heart tissue homogenate	1
10% Rats lung tissue homogenate	1
Human serum	1
1×10^6 Molt-4 cells	1
1×10^6 HL-60 cells	1
1×10^6 Jurkat cells	1
1×10^6 Hela cells	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.