Succinyl-CoA Synthetase (SCS) Activity Assay Kit (E-BC-K906-M)
For research use only.
| Detection Principle | Succinyl-CoA synthetase (SCS) is a key enzyme in the citric acid cycle and an important metabolic intermediate in the biosynthesis of porphyrins, hemes, and ketones. SCS converts succinyl-coenzyme A (CoA-SH) to succinic acid. The reaction between CoA-SH and pyruvate produces chromogenic substances with a characteristic absorption peak at 450 nm. The activity of SCS can be calculated by measuring the change of absorbance value at 450 nm. |
| Sample Type | Tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (440-460 nm, optimum wavelength: 450 nm) |
| Research Area | Glycolysis And Lipid Metabolism , Tricarboxylic Acid（TCA）Cycle , Energy Metabolism |
| Other Reagents Required | PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.09 U/L |
| Detection Range | 0.09-20.72 U/L |
| Precision | inter-assay CV: 4.9-10.3% | intra-assay CV: 2.5-8.0% |
| Sample Volume | 20 μL(cell/tissue homogenate) |
| Assay Time | 1 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Rat heart tissue homogenate | 1 |
| 10% Rat liver tissue homogenate | 1 |
| 10% Rat kidney tissue homogenate | 1 |
| 10% Mouse brain tissue homogenate | 1 |
| 1 × 10^6 4T1 cells | 1 |
| 1 × 10^6 Molt-4 cells | 1 |
| 1 × 10^6 Jurkat cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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