Chlorine (Cl) Colorimetric Assay Kit (E-BC-K189-M)
For research use only.
| Detection Principle | Chloride ion in biological fluids are replaced by the mercury ions in mercury thiocyanate through ion replacement, which resulted in the formation of difficult-to-dissociate mercury chloride. The substituted thiocyanate ions were combined with ferric nitrate to form a red complex. The content of chlorine ion can be calculated indirectly by measuring the OD value at 460 nm. |
| Synonyms | Cl |
| Sample Type | Serum,Plasma,Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (440-480 nm, optimum wavelength: 460 nm) |
| Research Area | Inorganic Ions , Plant Physiology Series |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 1 mmol/L |
| Detection Range | 1.0-60 mmol/L |
| Precision | inter-assay CV: 6.4% | intra-assay CV: 3.6% |
| Sample Volume | 10 μL |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenate | 2-3 |
| 10% Mouse kidney tissue homogenate | 2-3 |
| 10% Rat spleen tissue homogenate | 2-3 |
| Human serum | 5-10 |
| Mouse serum | 5-10 |
| Cynomolgus monkey serum | 5-10 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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