Magnesium (Mg) Colorimetric Assay Kit (E-BC-K162-M)
For research use only.
| Detection Principle | The magnesium in the serum reacts with the complexometric indicator (Calmagite) to form the Calmagite-Mg compound. The absorbance of this compound at 540 nm is proportional to the concentration of magnesium in the sample. The concentration of magnesium can be calculated by measuring the OD value at 540 nm. |
| Synonyms | Mg |
| Sample Type | Serum,Plasma |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (520-550 nm, optimum wavelength: 540 nm) |
| Research Area | Inorganic Ions , Plant Physiology Series |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.18 mmol/L |
| Detection Range | 0.18-2.50 mmol/L |
| Precision | inter-assay CV: 7.9% | intra-assay CV: 5.1% |
| Sample Volume | 2.5 μL |
| Assay Time | 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Rat serum | 1 |
| Mouse serum | 1 |
| Porcine serum | 1 |
| Chicken serum | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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