Zinc (Zn) Colorimetric Assay Kit (E-BC-K137-M)
For research use only.
| Detection Principle | The zinc ion in the sample react with 5-Br-PADAP to produce the colored complex. The depth of color is directly proportional to the concentration of zinc ion. Zinc ion content can be calculated by measuring the OD values at 560 nm. |
| Synonyms | Zn |
| Sample Type | Serum,Plasma,Urine,Milk |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (545-575 nm) |
| Research Area | Inorganic Ions , Plant Physiology Series |
| Other Reagents Required | Deionized water |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.418 μmol/L |
| Detection Range | 0.748-46.2 μmol/L |
| Precision | inter-assay CV: 0.04 | intra-assay CV: 2.7% |
| Sample Volume | 50 μL |
| Assay Time | 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human urine | 1 |
| Human serum | 1 |
| Human milk | 1 |
| Rat serum | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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