Phosphorus (Pi) Colorimetric Assay Kit (Phospho Molybdate Method) (E-BC-K245-M)
For research use only.
| Detection Principle | Inorganic phosphorus react with molybdic acid to produce phosphomolybdic acid. Phosphomolybdic acid can be reduced to molybdenum blue under the action of reducing agent. And the molybdenum blue have a maximum absorption peak at 660 nm. The phosphorus content can be calculated indirectly be measuring the OD value at 660 nm. |
| Synonyms | Pi |
| Sample Type | Serum,Plasma,Tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (620-690 nm, optimum wavelength: 660 nm) |
| Research Area | Inorganic Ions , Plant Physiology Series |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.004 mmol/L |
| Detection Range | 0.004-2.0 mmol/L |
| Precision | inter-assay CV: 0.03 | intra-assay CV: 2.1% |
| Sample Volume | 0.1 mL |
| Assay Time | 40 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Mouse serum | 1 |
| Rat plasma | 1 |
| Human urine | 2-3 |
| 10% Rat liver tissue homogenate | 1 |
| 10% Mouse lung tissue homogenate | 1 |
| 10% Mouse brain tissue homogenate | 1 |
| 10% Rat muscle tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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