Revertose Colorimetric Assay Kit (DNS Method) (E-BC-K757-M)

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All	Size	Price	Qty
	96T	$ 420.00
	48T	$ 280.00

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Detection Principle	Revertose are sugars with reducing properties. In sugars, both monosaccharides containing free aldehyde or ketone groups and disaccharides containing free aldehyde groups are reductive. Revertose mainly include glucose, fructose, galactose, lactose, maltose, etc. Revertose are reduced to amino compounds after co-heating with 3, 5-dinitrosalicylic acid under alkaline conditions, and are reddish brown in alkaline solution. The amount of revertose is proportional to the depth of color of reddish-brown substances, so as to determine the content of revertose in samples.
Sample Type	Serum,Plasma,Animal and plant tissue
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (530-550 nm, optimum wavelength: 540 nm)
Research Area	Plant Physiology Series
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	0.036 mg/mL
Detection Range	0.036-1.0 mg/mL
Precision	inter-assay CV: 2.1-5.3% \| intra-assay CV: 1.0-2.9%
Sample Volume	30 μL
Assay Time	50 min

Cat.No.	Product Name	Sizes
E-BC-K041-M	Maltase Activity Assay Kit	96T
E-BC-K755-M	Total Carbohydrate Colorimetric Assay Kit	96T , 48T
E-BC-K756-M	Maltose Colorimetric Assay Kit	96T
E-BC-K866-M	Plant Soluble Sugar Colorimetric Assay Kit	96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
Human serum	1
10% Mouse liver tissue homogenate	3-10
10% Wheat tissue homogenate	15-30
10% Paddy tissue homogenate	15-30
10% Corn tissue homogenate	15-30
10% Pumpkin tissue homogenate	10-20

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.