Plant Soluble Sugar Colorimetric Assay Kit (E-BC-K866-M)

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	96T	$ 280.00
	48T	$ 180.00

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Detection Principle	Carbohydrate is one of the important components of plant corpus, and is also the main raw material of metabolism and storage material. The kit is used for the determination of soluble monosaccharides, oligosaccharides and polysaccharides. It has the advantages of high sensitivity, quick convenience and suitable for the determination of microscale samples. The detection principle is anthrone colorimetry. Carbohydrate react with anthrone to produce colored material, which has the maximum absorption peak at 620 nm. The soluble sugar content can be determined by measuring the absorbance value.
Sample Type	Plant tissue
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (610-630 nm, optimum wavelength: 620 nm)
Research Area	Plant Physiology Series
Other Reagents Required	Concentrated sulfuric acid, Ethyl acetate
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Valid Period	12 months
Detection Range	0.003-1.5 mg/mL
Precision	inter-assay CV: 5.3% \| intra-assay CV: 1.7%
Sample Volume	200 μL(tissue homogenate)
Assay Time	20 min

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
10% Mango tissue homogenate	150-250
10% Fresh jujube tissue homogenate	150-250
10% Grape tissue homogenate	150-200
10% Corn tissue homogenate	100-200
10% Apple tissue homogenate	100-150
10% Banana tissue homogenate	150-200
10% Cucumber tissue homogenate	20-50
10% Tomato tissue homogenate	30-60
10% Mushroom tissue homogenate	1
10% Carrot tissue homogenate	40-60

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.