Phytic Acid Assay Kit (E-BC-K869-M)
For research use only.
| Detection Principle | Phytic acid, also known as inositol hexaphosphate, is widely found in cereals, beans, and vegetables. The detection principle of this kit: Phytic acid generates inorganic phosphorus under the catalysis of enzyme. Under acidic conditions, inorganic phosphorus reacts with ammonium molybdate to produce blue molybdenum blue substance, which has a characteristic absorption peak at 700 nm. By measuring the content of inorganic phosphorus, the phytic acid content can be calculated. |
| Sample Type | Plant tissue,Liquid |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (690-705 nm, optimum wavelength: 700 nm) |
| Research Area | Plant Physiology Series |
| Storage | This product can be stored at 2-8°C/-20℃ for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 3.78 nmol/mL |
| Detection Range | 3.78-500 nmol/mL |
| Precision | inter-assay CV: 2.3-4.1% | intra-assay CV: 4.5-6.0% |
| Sample Volume | 240 μL |
| Assay Time | 3 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Tomato tissue homogenate | 1 |
| 10% Carota tissue homogenate | 1 |
| 10% Green pepper tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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