Phytase Assay Kit (E-BC-K870-M)
For research use only.
| Detection Principle |
Phytase also known as inositol hexaphosphatase, is a protein and sugar binding enzyme. Phytase can catabolize phytic acid to produce inorganic phosphorus and inositol, which greatly improves nutrient utilization rate of organisms. Natural phytase is widely found in plants, animal tissues and microorganisms. Phytase has a wide research value in the field of food production and animal husbandry. Under certain environmental conditions, phytase can decompose sodium phytate to produce inorganic phosphorus and inositol derivatives. Under acidic conditions, inorganic phosphorus and ammonium molybdate chromogenic agent react to produce a blue molybdenum blue substance, which has a characteristic absorption peak at 700 nm. Phytase activity can be calculated by measuring the content of inorganic phosphorus. |
| Sample Type | Animal and Plant tissue,Microorganism |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (690-710 nm, optimum wavelength: 700 nm) |
| Research Area | Plant Physiology Series |
| Other Reagents Required | Concentrated sulfuric acid (98%) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 1 U/mL |
| Detection Range | 1 - 170 U/mL |
| Precision | inter-assay CV: 3.2-4.5% | intra-assay CV: 3.6-5.0% |
| Sample Volume | 100 μL |
| Assay Time | 50 min |
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