Phosphoglucose Isomerase (PGI) Activity Colorimetric Assay Kit (E-BC-K770-M)
For research use only.
| Detection Principle |
Phosphoglucose Isomerase (PGI) is an important enzyme that can catalyze the interconversion between glucose-6-phosphate (G6P) and fructose-6-phosphate (F6P), and is involved in glycolysis and the pentose phosphate pathway. These processes help cells break down glucose for energy and generate key substances in other metabolic pathways. The detection principle of this kit is as follows: The PGI catalyze the substrate to produce the product, reacting with chromogenic agent to form the chromogenic substance, which has the maximum absorption at a wavelength of 450 nm. The PGI enzyme activity is calculated by measuring the OD value at 450 nm. |
| Sample Type | Serum,Plasma,Animal tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (445-460 nm, optimum wavelength: 450 nm) |
| Research Area | Glycolysis And Lipid Metabolism |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.78 U/L |
| Detection Range | 0.78-50.09 U/L |
| Precision | inter-assay CV: 6.5-9.9% | intra-assay CV: 2.0-2.7% |
| Sample Volume | 20 μL |
| Assay Time | 1 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 10-40 |
| Rat serum | 10-40 |
| Mouse serum | 10-40 |
| 10% Mouse liver tissue homogenate | 100-200 |
| 10% Mouse kidney tissue homogenate | 100-200 |
| 10% Mouse muscle tissue homogenate | 100-200 |
| 1×10^6 HL-60 cells | 10-20 |
| 1×10^6 293T cells | 10-20 |
| 1×10^6 Jurkat cells | 10-20 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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