6-Phosphogluconate Dehydrogenase (6-PGDH) Activity Colorimetric Assay Kit (E-BC-K1150-M)
For research use only.
| Detection Principle |
6-phosphogluconate dehydrogenase (6-PGDH) is the rate-limiting enzyme of the pentose phosphate pathway, which is responsible for the production of NADPH, energy balance, growth rate, and cell viability. 6-PGDH is also associated with a variety of tumor signaling pathways, including epidermal growth factor signaling pathway and nuclear factor E2 related factor signaling pathway. The principle of this kit is that the product generated by the 6-PGDH catalytic substrate reacts with the chromogenic agent to generate the chromogenic substance, which has the maximum absorption at 450 nm, and is calculated by measuring the OD value at 450 nm 6-PGDH enzyme activity. |
| Sample Type | Serum,Plasma,Animal and plant tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (440-460 nm, optimum wavelength: 450 nm) |
| Research Area | Glycolysis And Lipid Metabolism |
| Other Reagents Required | PBS(0.01 M，pH 7.4) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 1.26 U/L |
| Detection Range | 1.26-101.85 U/L |
| Precision | inter-assay CV: 5.9-9.4% | intra-assay CV: 2.9-4.6% |
| Sample Volume | 20 μL |
| Assay Time | 50 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenate | 1-10 |
| 10% Mouse muscle tissue homogenate | 1-10 |
| 10% Mouse heart tissue homogenate | 1-10 |
| 10% Mouse brain tissue homogenate | 1-10 |
| 10% Mouse kidney tissue homogenate | 2-10 |
| 10% Broccoli tissue homogenate | 1 |
| 10% Potato tissue homogenate | 1 |
| 10% Corn tissue homogenate | 1-10 |
| 1×10^6 Hela 60 cells | 2-10 |
| 1×10^6 293T cells | 8-25 |
| Rats plasma | 1 |
| Fetal bovine plasma | 1 |
| Human serum | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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