Glucose-6-phosphate (G6P) Colorimetric Assay Kit (E-BC-K011-M)
For research use only.
| Detection Principle | Glucose-6-phosphate (G6P) is a molecule generated by phosphorylation of hydroxyl groups on the sixth carbon of glucose under the catalysis of hexokinase. It is a common small molecule of sugar metabolism in cells and participates in biochemical pathways such as glycolysis and pentose phosphate pathway. In the first reaction of glycolysis, glucose is catalyzed by hexokinase to produce glucose-6-phosphate, which is then catalyzed by phosphoglucose isomerase to form fructose-6-phosphate to continue the other steps of glycolysis: In the pentose phosphate pathway, glucose-6-phosphate is the first substrate, and this process is also the main way to generate NADPH. In addition to these two metabolic pathways, glucose-6-phosphate can also be converted into glycogen or starch and stored. |
| Synonyms | G6P |
| Sample Type | Serum,Plasma,Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (450 nm) |
| Research Area | Glycolysis And Lipid Metabolism , Disulfidptosis , Energy Metabolism |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 5.6 μmol /L |
| Detection Range | 5.6-500 μmol/L |
| Precision | inter-assay CV: 4.3% | intra-assay CV: 2.1% |
| Sample Volume | 100 μL |
| Assay Time | 35 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human plasma | 1 |
| Mouse serum | 2-3 |
| Rat plasma | 1 |
| Porcine serum | 1 |
| 10% Rat spleen tissue homogenate | 2-3 |
| 10% Rat heart tissue homogenate | 2-3 |
| 10% Rat liver tissue homogenate | 2-3 |
| 10% Mouse lung tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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