DPPH Free Radical Scavenging Capacity Colorimetric Assay Kit (E-BC-K807-M)
For research use only.
| Detection Principle | DPPH is a synthetic organic free radical that can be used to evaluate the antioxidant activity of antioxidants. Its single electron has the maximum absorption at 525 nm, and the DPPH radical scavenging ability of the sample can be calculated by measuring the change of OD value before and after the addition of antioxidants. |
| Sample Type | Serum,Plasma,Tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (505-545 nm, optimum wavelength: 525 nm) |
| Research Area | Oxidative Stress |
| Other Reagents Required | Anhydrous ethanol, 80% Ethanol |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 17.39 μmol VC/L |
| Detection Range | 17.39-125 μmol VC/L |
| Precision | inter-assay CV: 2.1-9.5% | intra-assay CV: 0.8-3.8% |
| Sample Volume | 800 μL(serum/plasma), 180 μL(tissue/cell homogenate) |
| Assay Time | 40 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Horse serum | 1 |
| Dog plasma | 1 |
| Mouse serum | 1 |
| Rat serum | 1 |
| 10% Rat liver tissue homogenate | 3-6 |
| 10% Rat heart tissue homogenate | 1 |
| 10% Mouse heart tissue homogenate | 1 |
| 10% Cow liver tissue homogenate | 4-8 |
| 10% Mouse lung tissue homogenate | 3-6 |
| 10% Mouse stomach tissue homogenate | 3-6 |
| 1.92 × 10^6 CHO cells | 1 |
| 2.164 × 10^6 Molt-4 cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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