Hydroxyl Free Radical Scavenging Capacity Assay Kit (E-BC-K527-M)

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Resources

All	Size	Price	Qty
	96T	$ 260.00
	48T	$ 180.00

Bulk request >>

Add to cart

For research use only. Order now, ship in 3 days

Detection Principle	H₂O₂/Fe²⁺ can generate hydroxyl free radical through Fenton reaction. Salicylic acid can effectively capture the generated hydroxyl radical and react with it to generate colored substances 2, 3-dihydroxybenzoic acid. There is a characteristic absorption peak at 510 nm. After adding the substances with scavenging ability, the colored substances are reduced, thus measuring the hydroxyl radical scavenging ability of the sample according to the value of absorption value.
Sample Type	Serum,Plasma,Animal tissue
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (500-530 nm, optimum wavelength: 510 nm)
Research Area	Oxidative Stress
Other Reagents Required	Absolute ethanol
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Valid Period	12 months
Precision	inter-assay CV: 4.6% \| intra-assay CV: 4.1%
Sample Volume	20 μL
Assay Time	40 min

Cat.No.	Product Name	Sizes
E-BC-K001-M	Inhibition and Production of Superoxide Anionic Colorimetric Assay Kit(WST-1 Method)	96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
Human serum	1
Dog serum	1
Rat serum	1
Cynomolgus monkey serum	1
10% Rat spleen tissue homogenate	1
10% Mouse liver tissue homogenate	1
10% Rat kidney tissue homogenate	1
10% Rat lung tissue homogenate	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.