Total Antioxidant Status (TAS) Colorimetric Assay Kit (E-BC-K801-M)
For research use only.
| Detection Principle | ABTS is oxidized to green ABTS+ by appropriate oxidant, which can be reduced to colorless ABTS in the presence of antioxidants. The TAS of the sample can be determined and calculated by measuring the absorbance of ABTS+ at 660 nm. Trolox is an analog of VE and has a similar antioxidant state to that of VE. Trolox is used as a reference substance for total antioxidant status. |
| Synonyms | TAS |
| Sample Type | Serum,Plasma,Urine,Cellular supernatant,Animal tissue,Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (650-670 nm, optimum wavelength: 660 nm) |
| Research Area | Oxidative Stress |
| Other Reagents Required | 60% Ethanol |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.23 mmol Trolox Equiv./L |
| Detection Range | 0.23-2 mmol Trolox Equiv./L |
| Precision | inter-assay CV: 0.07 | intra-assay CV: 4.6% |
| Sample Volume | 10 μL |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenate | 1 |
| 10% Rat liver tissue homogenate | 1 |
| 10% Rat lung tissue homogenate | 1 |
| Molt4 cellar supernatant | 1 |
| Human urine | 8-10 |
| Mouse serum | 1 |
| Human serum | 1 |
| Human saliva | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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