Coenzyme A (CoA) Colorimetric Assay Kit (E-BC-K907-M)
For research use only.
| Detection Principle |
Coenzyme A (CoA) is composed of units derived from cysteine, pantothenic acid, and ATP. It plays an important role in the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle, and other biological processes. The enzymatic reaction of Coenzyme A produces NADH, and the chromogenic substance produced by reaction with the chromogenic agent has a characteristic absorption peak at 450 nm. The content of CoA in the sample can be determined by detecting the absorbance. |
| Sample Type | Serum,Plasma,Animal tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (440-460 nm, optimum wavelength: 450 nm) |
| Research Area | Tricarboxylic Acid（TCA）Cycle , Energy Metabolism |
| Other Reagents Required | 1 mol/L Perchloric acid, 3 mol/L KHCO<sub>3</sub> |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.005 mmol/L |
| Detection Range | 0.005-0.5 mmol/L |
| Precision | inter-assay CV: 0.8-2.2% | intra-assay CV: 0.6-7.5% |
| Sample Volume | 50 μL |
| Assay Time | 50 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum (plasma) | 1 |
| Mouse serum (plasma) | 1 |
| 10% Mouse lung tissue homogenate | 1 |
| 10% Mouse liver tissue homogenate | 1 |
| 10% Mouse heart tissue homogenate | 1 |
| 10% Mouse brain tissue homogenate | 1 |
| 1×10^6 293T cell homogenate | 1 |
| 1×10^6 Molt-4 cell homogenate | 1 |
| 1×10^6 HL-60 cell homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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