Branched Chain Amino Acids (BCAA) Colorimetric Assay Kit (E-BC-K847-M)
For research use only.
| Detection Principle |
Branched Chain Amino Acids (BCAA), including leucine, isoleucine and valine, are essential amino acids for the human body, which have the functions of muscle synthesis, anti-protein breakdown and stimulating insulin production. The detection principle of this kit is: the branched chain amino acid under the catalysis of enzymes to generate the product of reaction with chromogenic agent generated color material, which has maximum absorption at 450 nm. The content of BCAA in samples was calculated by measuring OD value at 450 nm and standard curve. |
| Sample Type | Serum,Plasma,Animal and plant tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (440-460 nm, optimum wavelength: 450 nm) |
| Research Area | Amino Acids And Proteins |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.009 mmol/L |
| Detection Range | 0.009-2 mmol/L |
| Precision | inter-assay CV: 3.4-8.5% | intra-assay CV: 0.6-1.0% |
| Sample Volume | 10 μL |
| Assay Time | 1 h 10 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| 10% Mouse liver tissue homogenate | 5-10 |
| 10% Mouse kidney tissue homogenate | 5-10 |
| 10% Mouse heart tissue homogenate | 5-10 |
| 10% Mouse brain tissue homogenate | 1 |
| 10% Mouse spleen tissue homogenate | 1 |
| 1×10^6 Jurkat cells | 1 |
| 1×10^6 HL-60 cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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