β-Hydroxybutyrate (Ketone Body) Colorimetric Assay Kit (E-BC-K785-M)
For research use only.
| Detection Principle | β-hydroxybutyrate (β-HB), C4H8O3, accounts for about 75% of the total ketone body. Patients with diabetic ketoacidosis have increased production of NADH, which promotes the conversion of acetoacetic acid into β-HB. Therefore, the level of β-hydroxybutyrate can be used as an index to evaluate glycosuria. β-hydroxybutyrate dehydrogenase can catalyze the oxidative dehydrogenation of β-HB. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The content of β-HB can be calculated by measuring the change of absorbance value at 450 nm. |
| Synonyms | β-HB |
| Sample Type | Serum,Plasma,Urine,Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (440-460 nm, optimum wavelength: 450 nm) |
| Research Area | Metabolic Diseases , Glycolysis And Lipid Metabolism , Energy Metabolism |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.01 mmol/L |
| Detection Range | 0.01-2.00 mmol/L |
| Precision | inter-assay CV: 4.0% | intra-assay CV: 2.0% |
| Sample Volume | 10 μL |
| Assay Time | 1 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Human plasma | 1 |
| Rat /Mouse serum | 1 |
| Rat /Mouse plasma | 1 |
| 10% Rat liver tissue homogenate | 1 |
| 10% Rat kidney tissue homogenate | 1 |
| 10% Rat heart tissue homogenate | 1 |
| Human urine | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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