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Triglyceride(TG)Fluorometric Assay Kit (E-BC-F033)

All Size Price Qty
96T $ 520.00
48T $ 356.00
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For research use only.

Detection Principle Triglyceride is converted by enzyme to produce hydrolytic products, which is catalyzed by enzymes to produce fluorescent substances. The content of triglyceride in samples can be calculate by measuring the fluorescence value.
Synonyms TG
Sample Type Serum,Plasma,Tissue,Cell sample
Detection Method Fluorometric method
Detection Instrument Fluorescence microplate reader (Ex/Em=535 nm/587 nm)
Research Area Glycolysis And Lipid Metabolism ,  Energy Metabolism
Storage This product can be stored at 2-8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.
Valid Period 12 months
Sensitivity 0.8 μmol/L
Detection Range 0.8-30 μmol/L
Precision inter-assay CV: 9.1% | intra-assay CV: 3.9%
Sample Volume 20 μL
Assay Time 30 min

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
10% Porcine heart tissue homogenate 3-6
10% Porcine liver tissue homogenate 8-10
1 × 10^6 CHO cells 1-2
1 × 10^6 293T cells 1-2
Human serum 100-120
Mouse serum 100-120

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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Biotin

Chromogenic Biotin

Cyanine 3

Cyanine 5

Cyanine 5.5

Cyanine 7

Elab Fluor® Violet 450

Elab Fluor®430

Elab Fluor®488

Elab Fluor®568

Elab Fluor®594

Elab Fluor®647

Elab Fluor®680

Elab Fluor®700

Elab Fluor®750

Elab Fluor®Red 780

Elab Fluor®Violet 450

FITC

HRP

NHS-Biotin

NHS-LC-LC-Biotin

Sulfo-NHS-Biotin

Sulfo-NHS-LC-LC-Biotin

Unconjugated

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detection instrument

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