Low-Density Lipoprotein Cholesterol (LDL-C) Colorimetric Assay Kit (Double Reagents) (E-BC-K205-M)
For research use only.
| Detection Principle | Lipoproteins (except LDL) such as HDL, CM, and VLDL change structure and dissociate under the action of surfactants. The released micronized cholesterol molecules react with cholesterol enzyme reagents, and the generated hydrogen peroxide is trapped in the absence of coupling agent. It is consumed without color development. At this time, the LDL particles are still intact, and then the reagent containing coupling agent is added, which can dissociate the LDL particles to release cholesterol, which is catalyzed by cholesterol esterase (CE) and cholesterol oxidase (CO) and produce hydrogen peroxide. Hydrogen peroxide is catalyzed by oxidase (POD) in the presence of 4-aminoantipyrine (4-AA) and phenol (T-OOS) to form a red quinone compound. Because the cholesterol molecules of other lipoproteins have been removed, the shade is proportional to the amount of LDL-C. |
| Synonyms | LDL-C |
| Sample Type | Serum,Plasma,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (530-570 nm, optimum wavelength: 546 nm) |
| Research Area | Metabolic Diseases , Glycolysis Lipid Metabolism |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4), Isopropanol (AR) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.04 mmol/L |
| Detection Range | 0.04-12 mmol/L |
| Precision | inter-assay CV: 0.1 | intra-assay CV: 5.5% |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Human plasma | 1 |
| Mouse serum | 1 |
| Rat plasma | 1 |
| Porcine serum | 1 |
| 10% Mouse kidney tissue homogenate | 1 |
| 10% Rat liver tissue homogenate | 1 |
| HepG2 cell | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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