Total Phosphodiesterase (PDEs) Acitivity Colorimetric Assay Kit (E-BC-K782-M)
For research use only.
| Detection Principle |
Phosphodiesterase (PDEs) hydrolyzes the intracellular second messenger (cyclic adenosine phosphate or cyclic guanosine phosphate), thereby ending the biochemical effects of these second messengers. The principle of this kit is that by hydrolyzing the substrate with total PDEs, the resulting substance has a maximum absorption peak at 405 nm. The OD value at 405 nm and the standard curve were measured to calculate the total PDEs activity in the sample. |
| Sample Type | Serum,Plasma,Animal tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (400-410 nm, optimum wavelength: 405 nm) |
| Research Area | Glycolysis And Lipid Metabolism |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.16 U/L |
| Detection Range | 0.16-16.64 U/L |
| Precision | inter-assay CV: 4.1-5.6% | intra-assay CV: 1.2-3.0% |
| Sample Volume | 40 μL |
| Assay Time | 1 h 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human plasma | 1 |
| Rabbit serum | 1 |
| 10% Mouse liver tissue homogenate | 1 |
| 10% Mouse kidney tissue homogenate | 1-2 |
| 10% Mouse small intestine tissue homogenate | 1 |
| 10% Mouse heart tissue homogenate | 2-3 |
| 1×10^6 HL-60 cells | 1 |
| 1×10^6 293T cells | 1 |
| 1×10^6 Hela cells | 1 |
| 1×10^6 Molt-4 cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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