Phospholipase C (PLC) Activity Colorimetric Assay Kit (E-BC-K781-M)
For research use only.
| Detection Principle |
Phospholipase C (PLC) is a hydrolase that breaks down phosphatidylinositol diphosphate (PIP2) to form diacylglycerol (DAG) and inositol triphosphate (IP3). PLCS are widely found in bacteria, plants and animals. Its abnormal expression is associated with a variety of cancers, and studying the PLC mechanism can help develop new anticancer therapies. PLC also play a key role in nerve signaling and protection, helping to understand and treat neurodegenerative diseases. In addition, PLC plays an important role in insulin signaling and lipid metabolism, making it a hot topic in diabetes and obesity research. PLC catalyzes the substrate to produce chromogenic substance has maximum absorption at 410 nm, and the PLC enzyme activity is calculated by measuring OD value at 410 nm. |
| Sample Type | Serum,Plasma,Animal and plant tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (405-415 nm, optimum wavelength: 410 nm) |
| Research Area | Glycolysis And Lipid Metabolism |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.383 U/L |
| Detection Range | 0.383-16.588 U/L |
| Precision | inter-assay CV: 6.7-9.9% | intra-assay CV: 1.8-3.0% |
| Sample Volume | 20 μL |
| Assay Time | 1 h 10 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Mouse serum | 2-5 |
| Rat serum | 2-5 |
| Human serum | 2-5 |
| 10% Mouse liver tissue homogenate | 1 |
| 10% Mouse kidney tissue homogenate | 2-5 |
| 10% Mouse heart tissue homogenate | 1 |
| 10% Mouse lung tissue homogenate | 1 |
| 10% Mung bean sprout tissue homogenate | 2-5 |
| 10% Edamame tissue homogenate | 2-5 |
| 10% Peanut tissue homogenate | 2-5 |
| 10% Broccoli tissue homogenate | 2-5 |
| 2×10^6 HL-60 cells | 1 |
| 1.8×10^6 Hela cells | 1 |
| 2×10^6 CHO cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
Other Clones
{{antibodyDetailsPage.numTotal}} Results
-
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.：{{item.cat}}
{{index}} {{goods_show_value}}
Other Formats
{{formatDetailsPage.numTotal}} Results
-
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.：{{item.cat}}
{{index}} {{goods_show_value}}
-
IF：{{item.impact}}
Journal：{{item.journal}} ({{item.year}})
DOI：{{item.doi}}Reactivity：{{item.species}}
Sample Type：{{item.organization}}
-
Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}
