Succinate Dehydrogenase (SDH) Activity Assay Kit (E-BC-K649-M)
For research use only.
| Detection Principle | SDH catalyzes the dehydrogenation of succinate to fumarate, with electron transport materials transferring electrons to 2, 6-dichlorophenol indiophenol (DCPIP). Then, the reduced DCPIP is reduced to the oxidized DCPIP, which has a characteristic absorption peak at 600 nm. Therefore, the activity of SDH can be quantified by measure the change OD value at 600 nm. |
| Synonyms | SDH |
| Sample Type | Animal tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (590-610 nm, optimum wavelength: 600 nm) |
| Research Area | Mitochondria Function , Tricarboxylic Acid（TCA）Cycle , Energy Metabolism |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4). |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.83 U/L |
| Detection Range | 0.83-65.42 U/L |
| Precision | inter-assay CV: 0.04 | intra-assay CV: 0.02 |
| Sample Volume | 20 μL(tissue/cell homogenate) |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Rat liver tissue homogenate | 1 |
| 10% Rat heart tissue homogenate | 1 |
| 10% Rat kidney tissue homogenate | 1 |
| 10% Mouse liver tissue homogenate | 1 |
| 10% Mouse heart tissue homogenate | 1 |
| 10% Mouse kidney tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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