NAD-Malate Dehydrogenase (NAD-MDH) Activity Assay Kit (E-BC-K561-M)
For research use only.
| Detection Principle |
Malate dehydrogenase widely exists in animals, plants, bacteria and other organisms, is one of the key enzymes in tricarboxylic acid cycle, catalyze between malic acid and oxaloacetic acid reversible conversion. MDH can be divided into NAD-dependent MDH and NADP-dependent MDH according to different coenzyme specificity. NAD-MDH usually exists in the cytoplasm and mitochondria of bacteria and eukaryotic cells. NAD-MDH catalyzes the conversion of substrate malic acid and NAD to oxaloacetic acid and NADH. NADH makes WST-8 orange under the action of electron coupling agent. The activity of NAD-MDH can be calculated by measuring the change of absorbance value at 450 nm. |
| Synonyms | NAD-MDH |
| Sample Type | Serum,Plasma,Animal tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (440-460 nm, optimum wavelength: 450 nm) |
| Research Area | Mitochondria Function , Tricarboxylic Acid（TCA）Cycle , Energy Metabolism |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 1.06 U/L |
| Detection Range | 1.06-46.7 U/L |
| Precision | inter-assay CV: 8.4% | intra-assay CV: 0.03 |
| Sample Volume | 20 μL |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Rat kidney tissue homogenate | 1 |
| 10% Rat spleen tissue homogenate | 1 |
| 10% Rat liver tissue homogenate | 1 |
| 10% Rat heart tissue homogenate | 1 |
| 10% Mouse liver tissue homogenate | 1 |
| 10% Mouse lung tissue homogenate | 1 |
| Mouse serum | 1 |
| Rat serum | 1 |
| Mouse plasma | 1 |
| Rat plasma | 1 |
| Human serum | 1 |
| Dog serum | 1 |
| HT29 cell | 1 |
| Molt-4 cell | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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