S-Adenosyl-Homocysteine Hydrolase (SAHH) Activity Colorimetric Assay Kit (E-BC-K841-M)
For research use only.
| Detection Principle |
In mammalian cells, S-adenosine homocysteine hydrolase (SAHH) is the only hydrolase involved in the hydrolysis of S-adenosine homocysteine (SAH) to homocysteine (Hcy). Excessive SAHH activity can hydrolyze SAH to generate excessive Hcy, thereby causing geriatric diseases such as Alzheimer's disease, hypercysteinemia, and coronary heart disease. Inhibiting SAHH activity can effectively alleviate the hydrolysis of SAH. The detection principle of this kit: The substrate is hydrolyzed by SAHH, and the product reacts with the chromogenic agent at room temperature to form a yellow colored product, which has the maximum absorption peak at 410 nm. The activity of SAHH can be calculated by detecting the change in absorbance at 410 nm. |
| Sample Type | Animal tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (405-415 nm, optimum wavelength: 410 nm) |
| Research Area | Amino Acids And Proteins |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 1.34 U/L |
| Detection Range | 1.34-37.14 U/L |
| Precision | inter-assay CV: 3.7-7.8% | intra-assay CV: 1.8-3.4% |
| Sample Volume | 40 μL (tissue/cell homogenate) |
| Assay Time | 1 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenate | 1 |
| 10% Mouse brain tissue homogenate | 1 |
| 10% Mouse lung tissue homogenate | 1 |
| 10% Mouse spleen tissue homogenate | 1 |
| 10% Mouse kidney tissue homogenate | 1 |
| 10% Mouse heart tissue homogenate | 1 |
| 10% Mouse leg muscle tissue homogenate | 1 |
| 1×10^6 293T cells | 1 |
| 1×10^6 Jurkat cells | 1 |
| 1×10^6 Molt-4 cells | 1 |
| 1×10^6 Hela cells | 1 |
| 1×10^6 HL-60 cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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