Homocysteine (Hcy) Colorimetric Assay Kit (E-BC-K854-M)
For research use only.
| Detection Principle | Oxidative Hcy is converted into free Hcy, which can convert NADH to NAD+ through cyclic catalysis of enzymes, resulting in a decrease of OD value at 340 nm. The Hcy content in the sample is calculated by the rate of decrease. |
| Synonyms | Hcy |
| Sample Type | Serum,Plasma,Urine |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (330-350 nm, optimum wavelength: 340 nm) |
| Research Area | Amino Acids And Proteins |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 1.95 μmol/L |
| Detection Range | 1.95-100.0 μmol/L |
| Precision | inter-assay CV: 0.09 | intra-assay CV: 1.7% |
| Sample Volume | 20 μL |
| Assay Time | 25 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Human urine | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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