Pyruvate Carboxylase (PC) Activity Assay Kit (E-BC-K608-M)
For research use only.
| Detection Principle |
Pyruvate carboxylate (PC) is a biotin-dependent enzyme that converts pyruvate to oxaloacetic acid by carboxylation. PC is widely found in animals, molds and yeasts, but it is absent from plants and most bacteria. In the tricarboxylic acid cycle, it is the main complementary reaction supplying oxoacetate and the first rate-limiting enzyme in the gluconeogenesis process. The product of the PC-catalyzed substrate reacts with the reducing agent. The reducing agent has a maximum absorbance at 340 nm, and the enzyme activity of PC is calculated by calculating the amount of reducing agent consumed in the reaction. |
| Sample Type | Serum,Plasma,Animal tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (340 nm) |
| Research Area | Glycolysis And Lipid Metabolism , Energy Metabolism |
| Other Reagents Required | Double distilled water, Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 6.43 U/L |
| Detection Range | 6.43-876.53 U/L |
| Precision | inter-assay CV: 3.9-9.7% | intra-assay CV: 0.6-1.4% |
| Sample Volume | 10 μL |
| Assay Time | 35 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenate | 90-110 |
| 10% Mouse kidney tissue homogenate | 90-110 |
| 10% Mouse heart tissue homogenate | 90-110 |
| 10% Mouse lung tissue homogenate | 70-80 |
| 10% Rat liver tissue homogenate | 90-110 |
| 10% Rat kidney tissue homogenate | 90-110 |
| 10% Rat heart tissue homogenate | 90-110 |
| Rat plasma | 10-20 |
| Mouse plasma | 10-20 |
| Rat serum | 10-20 |
| Mouse serum | 10-20 |
| 1.92 × 10^6 CHO cells | 2-4 |
| 0.99 × 10^6 293T cells | 2-4 |
| 1.44 × 10^6 4T1 cells | 2-4 |
| 1.58 × 10^6 Jurkat cells | 2-4 |
| 1.24 × 10^6 Molt-4 cells | 2-4 |
| 0.88 × 10^6 Hela cells | 2-4 |
| 1.36 × 10^6 Raw cells | 2-4 |
| 1.24 × 10^6 HL-60 cells | 2-4 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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