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| Detection Principle | Nitrate nitrogen can react with salicylic acid to produce nitrosalicylic acid in a strongly acidic environment. Nitrosalicylic acid is yellow under strong alkaline conditions, and the depth of color is proportional to the content at a wavelength of 410 nm. |
| Sample Type | plant tissue,Soil |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (405-415 nm, optimum wavelength: 410 nm) |
| Research Area | Plant Physiology Series |
| Other Reagents Required | Potassium nitrate, Concentrated sulfuric acid |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 1.09 μg/mL |
| Detection Range | 1.09-200 μg/mL |
| Precision | inter-assay CV: 3.2-5.1% | intra-assay CV: 1.5-4.3% |
| Sample Volume | 10 μL(tissue homogenate) |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 20% Epipremnum aureum root tissue homogenization | 1 |
| 20% Soil homogenization | 1 |
| 20% Core leaves tissue homogenization | 1 |
| 20% Green pepper tissue homogenization | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
Other Clones
1 Results
Other Formats
1 Results
Biotin
Chromogenic Biotin
Cyanine 3
Cyanine 5
Cyanine 5.5
Cyanine 7
Elab Fluor® Violet 450
Elab Fluor®430
Elab Fluor®488
Elab Fluor®568
Elab Fluor®594
Elab Fluor®647
Elab Fluor®680
Elab Fluor®700
Elab Fluor®750
Elab Fluor®Red 780
Elab Fluor®Violet 450
FITC
HRP
NHS-Biotin
NHS-LC-LC-Biotin
Sulfo-NHS-Biotin
Sulfo-NHS-LC-LC-Biotin
Unconjugated
