Proline (Pro) Colorimetric Assay Kit (E-BC-K177-M)
For research use only.
| Detection Principle | Proline can react with acidic-ninhydrin to form stable red compound. The maximum absorption peak of the compound is at 520 nm. And the concentration of Pro can be calculated by measuring the OD value at 520 nm |
| Synonyms | Pro |
| Sample Type | Plant tissue,Honey sample |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (520-530 nm) |
| Research Area | Plant Physiology Series |
| Other Reagents Required | Acetic acid |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.57 μg/mL |
| Detection Range | 0.57-35 μg/mL |
| Precision | inter-assay CV: 4.7-10.0% | intra-assay CV: 3.7-5.5% |
| Sample Volume | 4 mL(honey/tissue homogenate) |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Green pepper tissue homogenate | 10-30 |
| Orange pulp tissue homogenate | 10-20 |
| Epipremnum aureum tissue homogenate | 1 |
| Carrot tissue homogenate | 1 |
| Garlic tissue homogenate | 1 |
| Pear tissue homogenate | 1 |
| Romaine lettuce tissue homogenate | 1 |
| Grape tissue homogenate | 1 |
| Yellow peach tissue homogenate | 1 |
| Plum tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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