Pectinase Activity Colorimetric Assay Kit (E-BC-K867-M)
For research use only.
| Detection Principle |
Pectinase is a class of pectin decomposition enzymes, including propectinase, pectin esterase, polygalacturonase and pectin lyase, which widely exist in plant fruits and microorganisms, and is the most important enzyme in fruit processing. The detection principle of this kit is that the substance generated by the hydrolysis of pectin by pectinase reacts with the chromogenic agent to produce a brown-red substance with characteristic absorption peak at 540 nm. The activity of pectinase can be calculated by measuring the change of absorbance value at 540 nm. |
| Sample Type | Liquid,Plant tissue,Microorganism |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (530-550 nm, optimum wavelength: 540 nm) |
| Research Area | Plant Physiology Series |
| Other Reagents Required | PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.05 U/mL |
| Detection Range | 0.05-20 U/mL |
| Precision | inter-assay CV: 4.5%-6.6% | intra-assay CV: 3.5-4.8% |
| Sample Volume | 80 μL |
| Assay Time | 1 h 30 min |
Other Clones
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Other Formats
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Biotin
Chromogenic Biotin
Cyanine 3
Cyanine 5
Cyanine 5.5
Cyanine 7
Elab Fluor® Violet 450
Elab Fluor®430
Elab Fluor®488
Elab Fluor®568
Elab Fluor®594
Elab Fluor®647
Elab Fluor®680
Elab Fluor®700
Elab Fluor®750
Elab Fluor®Red 780
Elab Fluor®Violet 450
FITC
HRP
NHS-Biotin
NHS-LC-LC-Biotin
Sulfo-NHS-Biotin
Sulfo-NHS-LC-LC-Biotin
Unconjugated
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