Cellulase (CL) Activity Colorimetric Assay Kit (E-BC-K1217-M)
For research use only.
| Detection Principle | Cellulase (CL) decomposed the substrate to produce raw sugar. Under the action of the enzyme, it formed colored substances with the chromogen. The activity of CL was measured by the absorbance at 505 nm. |
| Sample Type | Soil,Tissue bacteria,Bacterial supernatant,Other liquid |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (500-510 nm, optimum wavelength: 505 nm) |
| Research Area | Plant Physiology Series |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.92 U/g |
| Detection Range | 0.92-300 U/g |
| Precision | inter-assay CV: 4.5-5.3% | intra-assay CV: 3.5-4.9% |
| Sample Volume | 80 μL |
| Assay Time | 2 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Soil sample | 1 |
| 10% Decaying plant tissue homogenate | 1-10 |
| Bacteria supernatant | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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