Mitochondrial Complex Ⅴ(F0F1-ATPase/ATP Synthase) Activity Assay Kit (E-BC-K153-M)

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All	Size	Price	Qty
	96T	$ 520.00
	48T	$ 380.00

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Detection Principle	Mitochondrial complex V is also known as F₀F₁-ATP synthase. ATP is hydrolyzed by F₀F₁-ATP synthase to produce ADP, and ADP converts NADH into oxidized coenzyme I (NAD) after enzyme conversion reaction. Therefore, the activity of mitochondrial complex IV can be quantified by measure the change OD value at 340 nm.
Synonyms	ATP Synthase
Sample Type	Animal tissue
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (330-350 nm, optimum wavelength: 340 nm)
Research Area	Mitochondria Function , Cuproptosis , Energy Metabolism
Storage	This product can be stored at -20°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	3.55 U/L
Detection Range	12.13-74.21 U/L
Precision	inter-assay CV: 6.5% \| intra-assay CV: 5.6%
Sample Volume	40 μL(tissue/cell homogenate)
Assay Time	30 min

Cat.No.	Product Name	Sizes
E-BC-K149-M	Mitochondrial Complex I (NADH-CoQ Reductase) Activity Assay Kit	96T , 48T
E-BC-K150-M	Mitochondrial Complex II Activity Assay Kit	96T , 48T
E-BC-K151-M	Mitochondrial Complex Ⅲ (Coenzyme Q-Cytochrome C Reductase) Activity Assay Kit	96T , 48T
E-BC-K152-M	Mitochondrial Complex IV (Cytochrome C Oxidase ) Activity Assay Kit	96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
10% Mouse liver tissue homogenate	2-6
10% Rat liver tissue homogenate	1-4
10% Mouse kidney tissue homogenate	2-6
10% Rat muscle tissue homogenate	1-3
5×10^6 HL-60 cells	1-2
5 × 10^6 Jurkat cells	1-2

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.