Mitochondrial Complex II Activity Assay Kit (E-BC-K150-M)

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All	Size	Price	Qty
	96T	$ 480.00
	48T	$ 320.00

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Detection Principle	Coenzyme Q, the catalytic product of mitochondrial complex II, can further reduce 2, 6-dichloroindoxol, which has a characteristic absorption peak at 600 nm. Therefore, the activity of mitochondrial complex II can be quantified by measure the change OD value at 600 nm.
Sample Type	Animal tissue
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (590-610 nm, optimum wavelength: 600 nm)
Research Area	Mitochondria Function , Cuproptosis , Energy Metabolism
Other Reagents Required	Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4)
Storage	This product can be stored at -20°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	0.54 U/L
Detection Range	0.54-17.66 U/L
Precision	inter-assay CV: 0.05 \| intra-assay CV: 0.04
Sample Volume	190 μL(tissue/cell homogenate)
Assay Time	30 min

Cat.No.	Product Name	Sizes
E-BC-K138-F	Reactive Oxygen Species (ROS) Fluorometric Assay Kit (Green)	96T
E-BC-K152-M	Mitochondrial Complex IV (Cytochrome C Oxidase ) Activity Assay Kit	96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
10% Rat liver tissue homogenate	1
10% Rat heart tissue homogenate	1
10% Rat kidney tissue homogenate	1
10% Mouse liver tissue homogenate	1
10% Mouse kidney tissue homogenate	1
10% Mouse heart tissue homogenate	1
Jurkat cell	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.